References
Al-Rubeai, M.; Spier, R. E. Hybridoma cultivation in serum-supplemented and serum-free media. In: Spier, R. E.; Griffiths, J. B.; Stephenne, J.; Crooy, P. J., ed. Advances in animal cell biology and technology for bioprocesses. London: Butterworth; 1988:277–279.
Carswell, E. A.; Old, L. J.; Kassel, R. L., et al. An endotoxin-induced serum factor that causes necrosis of tumors. Proc. Natl. Acad. Sci. USA 72:3666–3670; 1975.
Chouaib, S.; Bertoglio, J.; Blay, J. Y., et al. Generation of lymphokine-activated killer cells: synergy between tumor necrosis factor and interleukin 2. Proc. Natl. Acad. Sci. USA 85:6875–6879; 1988.
Fiers, W. Tumor necrosis factor. Characterization at the molecular, cellular and in vivo levels. FEBS Lett. 285:199–212; 1991.
Fujii, S.; Liu, Y.; Neda, H., et al. Augmented systemic immunity in mice implanted with tumor necrosis factor-α gene-transduced Meth-A cells. Jpn. J. Cancer Res. 85:315–324; 1994.
Fushimi, K.; Torigoe, K.; Yamauchi, H.; Furusako, S.; Kurimoto, M.; Namba, M. Establishment of a human fibroblast cell line producing tumor necrosis factor α (KMST-6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture. In Vitro Cell. Dev. Biol. 34:463–467; 1998.
Glassy, M. C.; Tharakan, J. P.; Chau, P. C. Serum-free media in hybridoma culture and monoclonal antibody production. Biotechnol. Bioeng. 32:1015–1028; 1988.
Hasegawa, A.; Yamashita, H.; Kondo, S., et al. Proteose peptone enhances production of tissue-type plasminogen activator from human diploid fibroblasts. Biochem. Biophys. Res. Commun. 150:1230–1236; 1988.
Hayter, P. M.; Curling, E. M.; Baines, A. J., et al. Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture. Appl. Microbiol. Biotechnol. 34:559–564; 1991.
Jahan, I.; Mihara, K.; Bai, L., et al. Neoplastic transformation and characterization of human fibroblasts by treatment with 60Co gamma rays and the human c-Ha-ras oncogene. In Vitro Cell. Dev. Biol. 29A:763–767; 1993.
Jan, D. C.-H.; Jones, S. J.; Emery, A. N., et al. Peptone, a low-cost growth-promoting nutrient for intensive animal cell culture. Cytotechnology 16:17–24; 1994.
Kaufman, R. J.; Sharp, P. A. Evolution of chromosomal regions containing transfected and amplified dihydrofolate reductase sequences. Mol. Cell Biol. 3:699–711; 1983.
Keen, M. J.; Rapson, N. T. Development of a serum-free culture medium for the large scale production of recombinant protein from a Chinese hamster ovary cell line. Cytotechnology 17:153–163; 1995.
Namba, M.; Nishitani, K.; Hyodoh, F., et al. Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with 60Co gamma rays. Int. J. Cancer 35:275–280; 1985.
Østensen, M. E.; Thiele, D. L.; Lipsky, P. E. Tumor necrosis factor-α enhances cytolytic activity of human natural killer cells. J. Immunol. 138:4185–4191; 1987.
Pfizenmaier, K.; Scheurich, P.; Schluter, C., et al. Tumor necrosis factor enhances HLA-A, B, C and HLA-DR gene expression in human tumor cells. J. Immunol. 138:975–980; 1987.
Renard, J. M.; Spagnoli, R.; Mazier, C., et al. Evidence that monoclonal antibody production kinetics is related to the integral of viable cells in batch systems. Biotechnol. Lett. 10:91–97; 1988.
Savinell, J. M.; Lee, G. M.; Palsson, B. O. On the orders of magnitude of epigenic dynamics and monoclonal antibody production. Bioprocess Eng. 4:231–234; 1989.
Talmage, J. E.; Phollips, H.; Schneider, M., et al. Immunomodulatory properties of recombinant murine and human tumor necrosis factor. Cancer Res. 48:544–550; 1988.
Trill, J. J.; Shatzman, A. R.; Ganguly, S. Production of monoclonal antibodies in COS and CHO cells. Curr. Opin. Biotechnol. 6:553–560; 1995.
Wölfel, T.; Herr, W.; Coulie, P., et al. Lysis of human pancreatic adenocarcinoma cells by autologous HLA-class I-restricted cytolytic T-lynphocyte (CTL) clones. Int. J. Cancer 54:636–644; 1993.
Zang, M.; Trautmann, H.; Gandor, C., et al. Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium. Bio/Technology 13:389–392; 1995.
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Oh, SH., Miyazaki, M. & Namba, M. Development of a serum-free medium for a human immortalized fibroblast cell line (KMST-6/TNF) producing tumor necrosis factor-α (TNF-α) and growth inhibitory effects of its conditioned medium on malignant cells in culture. In Vitro Cell.Dev.Biol.-Animal 37, 169–171 (2001). https://doi.org/10.1290/1071-2690(2001)037<0169:DOASFM>2.0.CO;2
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DOI: https://doi.org/10.1290/1071-2690(2001)037<0169:DOASFM>2.0.CO;2